A fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to the hydrophilic end of cholesterol

3-dodecanoyl-NBD Cholesterol – 500 µg

Brand:
Cayman
CAS:
1246303-05-8
Storage:
-20
UN-No:
De Minimis - 1170 / 3

C-12 NBD cholesterol is a fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to the hydrophilic end of cholesterol, separated by a 12-carbon spacer. This design allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer. This should model the behavior of cholesterol in membranes better than the previously used 25-NBD cholesterol, which positions NBD directly on the 25th carbon of cholesterol at the hydrophobic terminus. NBD has excitation/emission maxima of 465/535 nm, respectively. Fluorescently tagged lipids have been used to study their interactions with proteins, their utilization by cells and liposomes, and for the development of assays for lipid metabolism.{16838,12304,1683913590,14063}  

 

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Description

A fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to the hydrophilic end of cholesterol, separated by a 12-carbon spacer; allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer; NBD ex/em = 465/535 nm, respectively

Formal name: 12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-(3S,10R,13R)-3-methoxy-10,13-dimethyl-17-((R)-6-methylheptan-2-yl)-tetradecahydro-1H-cyclopenta[α]phenanthrene

Synonyms:  3-C12-NBD Cholesterol

Molecular weight: 747.1

CAS: 1246303-05-8

Purity: ≥98%

Formulation: A solution in ethanol

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Lipid Biochemistry|Sterol Lipids

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