A fluorescent probe for lipid-binding proteins; binds with high affinity to hydrophobic surfaces of proteins; ex/em = 350/520 (free) and 468-477 nM (bound)

1,8-ANS – 50 g

Brand:
Cayman
CAS:
82-76-8
Storage:
-20
UN-No:
Non-Hazardous - /

1,8-ANS is a fluorescent dye that binds with high affinity to hydrophobic surfaces of proteins.{14908,14913} 1,8-ANS has an excitation maximum of 350 nm. It has an emission maximum of 520 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FABP2) it has emission maxima of 468-477 nm. 1,8-ANS binds to low polarity regions of protein surfaces making it well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins, such as the binding of free fatty acids to FABPs.{14908,14913,14912} 1,8-ANS binds to FABP2 with a Kd value of approximately 9.7 µM at 24.5°C.{14913}  

 

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Description

A fluorescent probe for lipid-binding proteins; binds with high affinity to hydrophobic surfaces of proteins; ex/em = 350/520 (free) and 468-477 nM (bound), respectively; undergoes a blue shift with an increase in fluorescence intensity when bound to protein; well suited for determining the affinity of hydrophobic ligands to their corresponding binding proteins; binds to FABP2 with a Kd value of approximately 9.7 µM at 24.5°C

Formal name: 8-(phenylamino)-1-naphthalenesulfonic acid

Synonyms:  1-Anilinonaphthalene-8-Sulfonic Acid

Molecular weight: 299.3

CAS: 82-76-8

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Lipid Biochemistry|Fatty Acids

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