A phenazine pigment and virulence factor produced by P. aeruginosa; increases ROS production in RAW 264.7 cells in a concentration-dependent manner; increases release of elastase and MPO by fMLP- and cytochalasin B-activated human neutrophils at ≥1.55 and ≥3.12 μM

1-Hydroxyphenazine – 100 mg

Brand:
Cayman
CAS:
528-71-2
Storage:
-20
UN-No:
Non-Hazardous - /

1-Hydroxyphenazine is a phenazine pigment and virulence factor produced by P. aeruginosa.{42947,42948} It increases production of reactive oxygen species (ROS) in RAW 264.7 cells in a concentration-dependent manner.{42948} 1-Hydroxyphenazine (≥1.55 and ≥3.12 μM) increases release of elastase and myeloperoxidase (MPO), respectively, by human neutrophils activated with N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP; Item No. 21495) and cytochalasin B (Item No. 11328).{42947} It reduces ciliary beat frequency in isolated guinea pig tracheal rings, resulting in ciliary dyskinesia by 1 hour when used at concentrations of 10, 50, and 100 μM and ciliary stasis by 5 hours at 50 and 100 μM.{42949} 1-Hydroxyphenazine reduces tracheal mucus velocity in anesthetized guinea pigs when administered as a 100 or 200 ng bolus dose. It is also active against the plant pathogenic fungi F. graminearum, P. grisea, A. solani, F. oxysporium, and S. sclerotiorum (MICs = 2-50 μg/ml).{42950}  

 

Available on backorder

Add to quotes
SKU: 28064 - 100 mg Category:

Description

A phenazine pigment and virulence factor produced by P. aeruginosa; increases ROS production in RAW 264.7 cells in a concentration-dependent manner; increases release of elastase and MPO by fMLP- and cytochalasin B-activated human neutrophils at ≥1.55 and ≥3.12 μM, respectively; reduces ciliary beat frequency in isolated guinea pig tracheal rings, resulting in ciliary dyskinesia by 1 hour at 10, 50, and 100 μM and ciliary stasis by 5 hours at 50 and 100 μM; reduces tracheal mucus velocity in anesthetized guinea pigs when administered as a 100 or 200 ng bolus dose; active against the plant pathogenic fungi F. graminearum, P. grisea, A. solani, F. oxysporium, and S. sclerotiorum (MICs = 2-50 μg/ml)

Formal name: 1-phenazinol

Synonyms:  Hemipyocyanine|NSC 88882

Molecular weight: 196.2

CAS: 528-71-2

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Natural Products|Microbial Metabolites||Product Type|Biochemicals|Ox Stress Reagents|Free Radical Generators||Product Type|Biochemicals|Pesticides|Fungicides||Research Area|Immunology & Inflammation|Innate Immunity||Research Area|Infectious Disease|Bacterial Diseases||Research Area|Oxidative Stress & Reactive Species|Pro-oxidant Activity||Research Area|Oxidative Stress & Reactive Species|Reactive Oxygen

Related products

  • CAY10512 is a substituted trans-stilbene analog of resveratrol that is 100-fold more potent as measured by antioxidant activity. The IC50 value for inhibition of TNFα-induced activation of NF-κB by CAY10512 is 0.15 µM compared to 20 µM by resveratrol.{14545}

    CAY10512 – 500 mg

    The nuclear factor-κB (NF-κB) regulates the expression of numerous genes involved in immunity and inflammation, cellular stress responses, growth, and apoptosis. Resveratrol, a polyphenolic trans-stilbene, is a known inhibitor of the activation of NF-κB and exhibits activity against a wide variety of cancer cells. CAY10512 is a substituted trans-stilbene analog of resveratrol that is 100-fold more potent as measured by antioxidant activity. The IC50 value for inhibition of TNFα-induced activation of NF-κB by CAY10512 is 0.15 µM compared to 20 µM by resveratrol.{14545}  

     

    Brand:
    Cayman
    SKU:10009536 - 500 mg

    Available on backorder

    Add to quotes
    Add to quotes
    View product page
  • A powerful effector in both the TetR and revTetR transcriptional regulator systems

    Anhydrotetracycline (hydrochloride) – 50 mg

    The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression.{17508} Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.{17509,17510} Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.{17508,17511} Moreover, anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet,{17512} so it does not act as a general inhibitor of translation and is a poor antibiotic. Perhaps related to this, the concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.{17508}  

     

    Brand:
    Cayman
    SKU:10009542 - 50 mg

    Available on backorder

    Add to quotes
    Add to quotes
    View product page
  • PtdIns-(3

    PtdIns-(3,4,5)-P3-biotin (sodium salt) – 50 µg

    The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.{8344,4096,14518} PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.  

     

    Brand:
    Cayman
    SKU:10009531 - 50 µg

    Available on backorder

    Add to quotes
    Add to quotes
    View product page
  • A powerful effector in both the TetR and revTetR transcriptional regulator systems

    Anhydrotetracycline (hydrochloride) – 500 mg

    The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression.{17508} Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.{17509,17510} Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.{17508,17511} Moreover, anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet,{17512} so it does not act as a general inhibitor of translation and is a poor antibiotic. Perhaps related to this, the concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.{17508}  

     

    Brand:
    Cayman
    SKU:10009542 - 500 mg

    Available on backorder

    Add to quotes
    Add to quotes
    View product page