Cayman
Showing 23851–24000 of 45550 results
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Antigen: histone 3 peptide with citrullinations at R2, R8, and R17 • Isotype: IgG1 • Host: rabbit • Species Reactivity: (+) human • Application(s): ELISA and WB
Brand:CaymanSKU:17939- 100 µg Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. The basic structure is a 146 bp strand of DNA wrapped around a histone octamer containing pairs of the four core histones (H2A, H2B, H3, and H4). Histones are subjected to numerous post-translational modifications including citrullination (de-imination). Citrullination is necessary for the development of neutrophil extracellular traps (NETs) (hyper-citrullination of histones by PAD4).{28015,28019} NETs are a part of the inflammatory response, and neutrophils use NETs to trap and eradicate bacteria and fungi. Failure to clear citrullinated proteins and NET components following inflammation can result in the production of autoantibodies and anti-citrullinated protein antibodies.{28017} The persistence of these antibodies and citrullinated proteins is associated with a number of human diseases including rheumatoid arthritis, systemic lupus erythematosus, Alzheimer’s disease, and multiple sclerosis.{28018} The ability to effectively detect the presence of citrullinated proteins is difficult and presents a barrier to further the understanding of these pathologies. Cayman’s Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody detects citrullinated human H3 by western blot, and does not detect unmodified H3.
Brand:CaymanSKU:17939 - 100 µgAvailable on backorder
Immunogen: Histone H3 peptide with citrullinations at R2, R8, and R17 • Host: Rabbit • Species Reactivity: (+) Human; other species not tested • Cross Reactivity: (-) Non-citrullinated histone H3 • Applications: ELISA, WB • MW = 16 kDa
Brand:CaymanSKU:17855- 1 ea Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.{56209} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma.{56209,30129,35770,46253} Cayman’s Histone H3 (Citrullinated R2 + R8 + R17) Polyclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes citrullinated histone H3 at 16 kDa from human samples.
Brand:CaymanSKU:17855 - 1 eaAvailable on backorder
Immunogen: Histone H3 peptide with citrullinations at R2, R8, and R17 • Host: Rabbit • Species Reactivity: (+) Human; other species not tested • Cross Reactivity: (-) Non-citrullinated histone H3 • Applications: ELISA, WB • MW = 16 kDa
Brand:CaymanSKU:17855- 1 eaAvailable on backorder
Brand:CaymanSKU:32937 - 100 µlAvailable on backorder
Brand:CaymanSKU:32937- 100 µlAvailable on backorder
Brand:CaymanSKU:32937- 100 µl
Antigen: synthetic peptide from human histone H3 • Host: mouse, clone 117C826 • Cross-Reactivity: (+) Human Histone H3 • Application(s): WB • H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.
Brand:CaymanSKU:13540- 1 eaAvailable on backorder
Antigen: synthetic peptide from human histone H3 • Host: mouse, clone 117C826 • Cross-Reactivity: (+) Human Histone H3 • Application(s): WB • H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.
Brand:CaymanSKU:13540- 1 ea The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation, and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.
Brand:CaymanSKU:13540 - 1 eaAvailable on backorder
Brand:CaymanSKU:32941 - 100 µlAvailable on backorder
Brand:CaymanSKU:32941- 100 µlAvailable on backorder
Brand:CaymanSKU:32941- 100 µl
Brand:CaymanSKU:32939 - 100 µ1Available on backorder
Brand:CaymanSKU:32939- 100 µ1Available on backorder
Brand:CaymanSKU:32939- 100 µ1
Brand:CaymanSKU:32940 - 100 µlAvailable on backorder
Brand:CaymanSKU:32940- 100 µlAvailable on backorder
Brand:CaymanSKU:32940- 100 µl
Brand:CaymanSKU:32936 - 100 µlAvailable on backorder
Brand:CaymanSKU:32936- 100 µlAvailable on backorder
Brand:CaymanSKU:32936- 100 µl
Immunogen: Recombinant histone H3 protein • Host: Mouse • Species Reactivity: (+) Human • Cross Reactivity: (-) Histones H2A, H2B, H4 • Applications: ELISA, WB • MW = 17 kDa
Brand:CaymanSKU:19824- 100 µgAvailable on backorder
Immunogen: Recombinant histone H3 protein • Host: Mouse • Species Reactivity: (+) Human • Cross Reactivity: (-) Histones H2A, H2B, H4 • Applications: ELISA, WB • MW = 17 kDa
Brand:CaymanSKU:19824- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Histone H3 PTMs function as epigenetic regulators of gene transcription by affecting chromatin structure and providing binding sites for many transcription factors, thus regulating several cellular functions including gene expression, cell cycle, and DNA replication and repair.{57056,56203} Differential methylation of histone H3 at various lysine residues is catalyzed by SET domain-containing methyltransferases and marks sites of transcriptional activation or repression.{57056} Citrullination of histone H3 by protein arginine deiminase 4 (PAD4; Item Nos. 10500 | 25915 | 28910) or PAD2 (Item No. 10785) induces the release of neutrophil extracellular traps (NETs), a network of decondensed DNA and intracellular proteins secreted by neutrophils as a pathogen defense mechanism.{52711,52712} Histone H3 mutations have been found in patients with diffuse intrinsic pontine glioma, leukemia, or chondroblastoma.{52713,49724} Cayman’s Histone H3 Monoclonal Antibody (Clone 2B8) can be used for ELISA and Western blot (WB) applications. The antibody recognizes histone H3 at 17 kDa from human samples.
Brand:CaymanSKU:19824 - 100 µgAvailable on backorder
Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Histone H3 PTMs function as epigenetic regulators of gene transcription by affecting chromatin structure and providing binding sites for many transcription factors, thus regulating several cellular functions including gene expression, cell cycle, and DNA replication and repair.{57056,56203} Differential methylation of histone H3 at various lysine residues is catalyzed by SET domain-containing methyltransferases and marks sites of transcriptional activation or repression.{57056} Citrullination of histone H3 by protein arginine deiminase 4 (PAD4; Item Nos. 10500 | 25915 | 28910) or PAD2 (Item No. 10785) induces the release of neutrophil extracellular traps (NETs), a network of decondensed DNA and intracellular proteins secreted by neutrophils as a pathogen defense mechanism.{52711,52712} Histone H3 mutations have been found in patients with diffuse intrinsic pontine glioma, leukemia, or chondroblastoma.{52713,49724} Cayman’s Histone H3 Polyclonal Antibody can be used for ELISA, flow cytometry (FC), immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes histone H3 at 15.5 kDa from human samples.
Brand:CaymanSKU:13781 - 500 µgAvailable on backorder
Immunogen: Recombinant histone H3 protein • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (-) H2A, H2B, H4 • Applications: ELISA, FC, IP, WB • MW = 15.5 kDa
Brand:CaymanSKU:13781- 500 µgAvailable on backorder
Immunogen: Recombinant histone H3 protein • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (-) H2A, H2B, H4 • Applications: ELISA, FC, IP, WB • MW = 15.5 kDa
Brand:CaymanSKU:13781- 500 µg
Immunogen: Peptide corresponding to human histone H3.3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3.3 independent of PTMs; (-) H3.1, other histone proteins • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32185- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to human histone H3.3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3.3 independent of PTMs; (-) H3.1, other histone proteins • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32185- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs). Metazoans have three histone variants: canonical histone H3.1, DNA replication-independent histone H3.3, and the centromeric variant CENP-A.{54380} Histone H3.3 is incorporated into chromatin in a DNA replication-independent manner at sites of active transcription.{54380,54382,54381} Mutations in H3F3A, the gene encoding histone H3.3 in humans, at or close to lysine 27 or glycine 34 induce upregulation of the oncogene MYCN and are correlated with poor survival in patients with pediatric glioblastoma.{54382} Cayman’s Histone H3.3 Monoclonal Antibody can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.
Brand:CaymanSKU:32185 - 100 µgAvailable on backorder
Histone H3.3 is a nuclear protein and a replication-independent member of the histone H3 family that has a role in nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Expression of H3F3A is common throughout the cell cycle independent of DNA synthesis.
Brand:CaymanSKU:13784 - 1 eaAvailable on backorder
Antigen: synthetic peptide from human Histone H3.3 amino acids 100-136 • Host: rabbit • Cross Reactivity: (+) chicken, ovine, Drosophila, equine, human, mouse, and opossum histone H3.3 • Application(s): IHC and WB • Histone H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Brand:CaymanSKU:13784- 1 eaAvailable on backorder
Antigen: synthetic peptide from human Histone H3.3 amino acids 100-136 • Host: rabbit • Cross Reactivity: (+) chicken, ovine, Drosophila, equine, human, mouse, and opossum histone H3.3 • Application(s): IHC and WB • Histone H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Brand:CaymanSKU:13784- 1 ea
Immunogen: An acetyl peptide corresponding to H3K14Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K79Ac; (-) Unmodified H3K79, H3K4Ac, H3K9Ac, H3K18Ac, H3K23Ac, H3K27Ac, H3K36Ac, H3K79Ac • Applications: ELISA, Multiplex-based assays, ICC, ChIP, WB
Brand:CaymanSKU:32129- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail, that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Acetylation of histone H3 at lysine 14 (H3K14) is associated with transcriptional activation and is required for trimethylation of H3K4.{45196,42735,42736} Cayman’s Histone H3K14Ac Monoclonal Antibody can be used for ELISA, multiplex-based assay, immunocytochemistry (ICC), chromatin immunoprecipitation (ChIP), and Western blot (WB) applications.
Brand:CaymanSKU:32129 - 100 µgAvailable on backorder
Immunogen: An acetyl peptide corresponding to H3K14Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K79Ac; (-) Unmodified H3K79, H3K4Ac, H3K9Ac, H3K18Ac, H3K23Ac, H3K27Ac, H3K36Ac, H3K79Ac • Applications: ELISA, Multiplex-based assays, ICC, ChIP, WB
Brand:CaymanSKU:32129- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K14Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K14Me2; (-) Unmodified H3K14, H3K14Me1, H3K14Me3, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32143- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K14Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K14Me2; (-) Unmodified H3K14, H3K14Me1, H3K14Me3, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32143- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Dimethylation of histone 3 lysine 14 (H3K14Me2) is enriched in the promoter region of tumor suppressor candidate 3 (TUSC3), but not that of GAPDH, in DLD-1 and HCT116 cells.{58054} Ubiquitin-like PHD and RING finger domain-containing protein 1 (UHRF1) can bind to dimethylated H3K14 and suppress acetylation of H3K14. H3K14Me2 levels increase during transdifferentiation of rat Thy-1(+) Lin(-) bone marrow cells into hepatocytes in vitro.{58055} Cayman’s Histone H3K14Me2 Monoclonal Antibody can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32143 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Ac; (-) H3K18, H3K4Ac, H3K9Ac, H3K14Ac, H3K23Ac, H3K27Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ChIP, ELISA, ICC, IHC, multiplex-based assay, WB
Brand:CaymanSKU:32140- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Ac; (-) H3K18, H3K4Ac, H3K9Ac, H3K14Ac, H3K23Ac, H3K27Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ChIP, ELISA, ICC, IHC, multiplex-based assay, WB
Brand:CaymanSKU:32140- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Acetylation of histone H3 at lysine 18 (H3K18Ac) by histone acetyltransferases (HATs) is associated with active gene transcription, whereas deacetylation of H3K18Ac by histone deacetylases (HDACs), including SIRT7, induces transcriptional repression.{21963} Tumor H3K18Ac hypoacetylation is associated with poor prognosis and increased risk of tumor recurrence in patients with lung adenocarcinoma or kidney clear cell carcinoma.{57202} Cayman’s Histone H3K18Ac Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32140 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Me1 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Me1; (-) Unmodified H3K18, H3K18Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32149- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Me1 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Me1; (-) Unmodified H3K18, H3K18Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32149- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Monomethylation of histone H3 at lysine 18 (H3K18Me1) has a slower turnover rate than all other histone H3 monomethylation sites with gene-activating function.{54352,56209} Cayman’s Histone H3K18Me1 Monoclonal Antibody can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.
Brand:CaymanSKU:32149 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Me2; (-) Unmodified H3K18, H3K4Me1, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32148- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K18Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K18Me2; (-) Unmodified H3K18, H3K4Me1, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32148- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Histone H3 lysine 18 (H3K18) is subject to both acetylation and methylation.{60019} An H3K18Me2 (1-21) peptide reduces the association of lamin A/C with chromatin in a chromatin immunoprecipitation (ChIP) assay.{60020} Dimethylation of histone H3 at lysine 18 is increased in patients with diabetes.{60021} Cayman’s Histone H3K18Me2 Monoclonal Antibody can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32148 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K23Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K23Ac; (-) Unmodified H3K23, H3K4Ac, H3K9Ac, H3K14Ac, H3K18Ac, H3K27Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32161- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K23Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K23Ac; (-) Unmodified H3K23, H3K4Ac, H3K9Ac, H3K14Ac, H3K18Ac, H3K27Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32161- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Acetylation of histone H3 at lysine 23 (H3K23Ac) is associated with transcriptional activation.{42735,42736} Levels of H3K23Ac are decreased in control, but increased in uninephrectomized, db/db diabetic mouse kidney compared with wild-type mouse kidney.{42737} Cayman’s Histone H3K23Ac Monoclonal Antibody can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32161 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K23Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K23Me2; (-) Unmodified H3K23, H3K23Me1, H3K4Me1, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB ,,
Brand:CaymanSKU:32150- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K23Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K23Me2; (-) Unmodified H3K23, H3K23Me1, H3K4Me1, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB ,,
Brand:CaymanSKU:32150- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Dimethylation of histone H3 at lysine 23 (H3K23Me2) is a predominant histone modification in C. elegans and is associated with heterochromatin and gene suppression.{60022,60023} Cayman’s Histone H3K23Me2 Monoclonal Antibody can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32150 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K27Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K27Ac; (-) Unmodified H3K27, H3K4Ac, H3K9Ac, H3K14Ac, H3K18Ac, H3K23Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32141- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K27Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K27Ac; (-) Unmodified H3K27, H3K4Ac, H3K9Ac, H3K14Ac, H3K18Ac, H3K23Ac, H3K36Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32141- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Acetylation of histone H3 at lysine 27 (H3K27Ac) is associated with active gene transcription.{46252} H3K27 acetylation and H3K4 monomethylation are enriched near active enhancers, and H3K27 acetylation and H3K4 trimethylation together mark transcription start sites of actively transcribed genes. Increased tumor H3K27Ac levels have been found in patients with breast cancer.{54329} Cayman’s Histone H3K27Ac Monoclonal Antibody can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32141 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K27Me3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K27Me3; (-) Unmodified histone H3K27, H3K27Me1, H3K27Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ChIP, ELISA, IHC, multiplex-based assays, WB
Brand:CaymanSKU:20719- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K27Me3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K27Me3; (-) Unmodified histone H3K27, H3K27Me1, H3K27Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K36Me1, H3K36Me2, H3K36Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ChIP, ELISA, IHC, multiplex-based assays, WB
Brand:CaymanSKU:20719- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Trimethylation of histone H3 at lysine 27 (H3K27Me3) is associated with gene silencing.{42743} It is involved in tumor progression through its regulation by enhancer of zeste homolog 2 (EZH2) and transcriptional repression of tumor suppressor genes.{49135,49132} Levels of H3K27Me3 are reduced in 293 T-REx cells containing EEDR236T and SUZ12G610V mutations and in lymphoblastoid cells isolated from patients with Weaver syndrome, a rare overgrowth disorder characterized by EZH2, EED, or SUZ12 mutations, cancer susceptibility, and various distinctive physical features.{49133} Cayman’s Histone H3K27Me3 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:20719 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K36Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Ac, H3 (Phospho-Ser31) K36Ac; (-) Unmodified H3K36, H3K4Ac, K3K9Ac, K3K14Ac, H3K18Ac, H3K23Ac, H3K27Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ChIP, ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32135- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K36Ac • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Ac, H3 (Phospho-Ser31) K36Ac; (-) Unmodified H3K36, H3K4Ac, K3K9Ac, K3K14Ac, H3K18Ac, H3K23Ac, H3K27Ac, H3K56Ac, H3K79Ac, H3K122Ac • Applications: ChIP, ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32135- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Acetylation of histone H3 at lysine 36 (H3K36Ac) is predominantly localized to the promoters of genes transcribed by RNA polymerase II and is highly conserved in mammals.{57199} Cayman’s Histone H3K36Ac Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32135 - 100 µgAvailable on backorder
Immunogen: A peptide corresponding to histone H3K36M mutant • Host: Rabbit • Cross Reactivity: (-) Wild-type histone H3 • Species Reactivity: (+) Histone H3K36M mutant • Applications: ELISA, IHC, WB • A probe for immunochemical detection of the H3K36M mutant
Brand:CaymanSKU:31547- 100 µl Histone H3 undergoes many modifications including acetylation, methylation, and phosphorylation that are important for regulation of gene transcription. Lysine 36 of histone 3 (H3K36) has functionally relevant acetylation and methylation states with roles in transcriptional activation. More than 90% of chondroblastomas contain a heterozygous mutation, replacing H3K36 with methionine 36 (H3K36M) in the histone H3 variant H3.3.{32234} This antibody reacts to the H3K36M mutant and does not detect wild-type histone H3.
Brand:CaymanSKU:31547 - 100 µlAvailable on backorder
Immunogen: A peptide corresponding to histone H3K36M mutant • Host: Rabbit • Cross Reactivity: (-) Wild-type histone H3 • Species Reactivity: (+) Histone H3K36M mutant • Applications: ELISA, IHC, WB • A probe for immunochemical detection of the H3K36M mutant
Brand:CaymanSKU:31547- 100 µlAvailable on backorder
Immunogen: A peptide corresponding to histone H3K36M mutant • Host: Rabbit • Cross Reactivity: (-) Wild-type histone H3 • Species Reactivity: (+) Histone H3K36M mutant • Applications: ELISA, IHC, WB • A probe for immunochemical detection of the H3K36M mutant
Brand:CaymanSKU:31547- 400 µl Histone H3 undergoes many modifications including acetylation, methylation, and phosphorylation that are important for regulation of gene transcription. Lysine 36 of histone 3 (H3K36) has functionally relevant acetylation and methylation states with roles in transcriptional activation. More than 90% of chondroblastomas contain a heterozygous mutation, replacing H3K36 with methionine 36 (H3K36M) in the histone H3 variant H3.3.{32234} This antibody reacts to the H3K36M mutant and does not detect wild-type histone H3.
Brand:CaymanSKU:31547 - 400 µlAvailable on backorder
Immunogen: A peptide corresponding to histone H3K36M mutant • Host: Rabbit • Cross Reactivity: (-) Wild-type histone H3 • Species Reactivity: (+) Histone H3K36M mutant • Applications: ELISA, IHC, WB • A probe for immunochemical detection of the H3K36M mutant
Brand:CaymanSKU:31547- 400 µlAvailable on backorder
Immunogen: Peptide corresponding to H3K36Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Me2; (-) Unmodified H3K36, H3K36Me1, H3K36Me3, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32133- 100 µgAvailable on backorder
Immunogen: Peptide corresponding to H3K36Me2 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Me2; (-) Unmodified H3K36, H3K36Me1, H3K36Me3, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32133- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Dimethylation of H3K36 is catalyzed by SET domain-containing histone methyltransferases including SET2, HYPB, NSD1, and ASH1L, and mutation of these methyltransferases is associated with various diseases, including multiple myeloma and Sotos syndrome.{57055,48281,20289} H3K36Me2 is enriched at the coding regions of genes and correlates with the initiation, but not maintenance, of active transcription, heterochromatin maintenance, and DNA break repair.{48281,48280,20250} Cayman’s Histone H3K36Me2 Monoclonal Antibody can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.
Brand:CaymanSKU:32133 - 100 µgAvailable on backorder
Immunogen: Synthetic peptide from human histone H3 amino acids 26-44 (dimethyl K36) conjugated to KLH • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (+) Histone H3 amino acids 26-44 (~10%), (+) Histone H3 dimethylated at lysine 9 (~10%), (+) Histone H3 monomethylated at lysine 36 (~10%), (+) Histone H3 trimethylated at lysine 36 (~10%) • Applications: ELISA and WB • MW = ~16 kDa
Brand:CaymanSKU:18200- 1 eaAvailable on backorder
Immunogen: Synthetic peptide from human histone H3 amino acids 26-44 (dimethyl K36) conjugated to KLH • Host: Rabbit • Species Reactivity: (+) Human • Cross Reactivity: (+) Histone H3 amino acids 26-44 (~10%), (+) Histone H3 dimethylated at lysine 9 (~10%), (+) Histone H3 monomethylated at lysine 36 (~10%), (+) Histone H3 trimethylated at lysine 36 (~10%) • Applications: ELISA and WB • MW = ~16 kDa
Brand:CaymanSKU:18200- 1 ea Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei.{57055} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various posttranslational modifications, including mono-, di-, and trimethylation at histone H3 lysine 36 (H3K36). Dimethylation of H3K36 is catalyzed by SET domain-containing histone methyltransferases including SET2, HYPB, NSD1, and ASH1L, and mutation of these methyltransferases is associated with various diseases, including multiple myeloma and Sotos syndrome.{57055,48281,20289} H3K36Me2 is enriched at the coding regions of genes and correlates with the initiation, but not maintenance, of active transcription, heterochromatin maintenance, and DNA break repair.{48280,48281,20250} Cayman’s Histone H3K36Me2 Polyclonal Antibody can be used for ELISA and Western blot applications. It detects H3K36Me2 at ~16 kDa from human samples.
Brand:CaymanSKU:18200 - 1 eaAvailable on backorder
Immunogen: A peptide corresponding to H3K36Me3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Me3; (-) Unmodified H3K36, H3K36Me1, H3K36Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ChIP, ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32134- 100 µgAvailable on backorder
Immunogen: A peptide corresponding to H3K36Me3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) H3K36Me3; (-) Unmodified H3K36, H3K36Me1, H3K36Me2, H3K4Me1, H3K4Me2, H3K4Me3, H3K9Me1, H3K9Me2, H3K9Me3, H3K14Me2, H3K18Me1, H3K18Me2, H3K23Me1, H3K23Me2, H3K27Me1, H3K27Me2, H3K27Me3, H3K56Me1, H3K79Me1, H3K79Me2, H3K79Me3 • Applications: ChIP, ELISA, multiplex-based assays, WB
Brand:CaymanSKU:32134- 100 µg Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Trimethylation of H3K36 is increased in the G1 and early S phases of the cell cycle where it binds to and recruits the mismatch recognition protein MutSα in preparation for DNA replication and mismatch repair.{46225} H3K36 trimethylation is found in greater amounts on exons compared with introns in C. elegans, mouse, and human genome-wide maps of histone H3 tail methylations.{46226} The loss of H3K36Me3 is correlated with poor prognosis in multiple cancer types while high levels increase expression of tumor suppressor genes.{56220} H3K36Me3 is negatively correlated with esophageal squamous cell carcinoma (ESCC) metastasis and positively correlated with increased survival in patients with ESCC. Cayman’s Histone H3K36Me3 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assay, and Western blot (WB) applications.
Brand:CaymanSKU:32134 - 100 µgAvailable on backorder
Immunogen: Peptide corresponding to the N-terminus of histone H3 • Host: Rabbit • Species Reactivity: (+) Vertebrates • Cross Reactivity: (+) Unmodified histone H3 (1-9), H3R2Me1 (1-9), H3R2Me2 (1-9), H3T3Ph (1-9); (-) H3K4Ac (1-9), H3K4Me1 (1-9), H3K4Me2 (1-9), H3K4Me3 (1-9) • Applications: ELISA, ICC, multiplex-based assays, WB
Brand:CaymanSKU:32160- 100 µgAvailable on backorder