Description
Boster’s Rat BMPER ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for BMPER has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: V34-R685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMPER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BMPER amount of sample captured in plate.
Synonyms: CV2; CV-2; CRIM3
Species: Rat BMPER
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant rat BMPER
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, V34-R685
Gene name: BMP-binding endothelial regulator / BMP-binding endothelial regulator
Protein name: BMP-binding endothelial regulator
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|226|813|1749|235|854|1806
Standard deviation|10.62|45.53|101.44|15.04|62.34|140.87
CV(%)|4.7%|5.6%|5.8%|6.4%|7.3%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 226 | 217 | 208 | 243 | 224 | 10.53 | 4.7% |
| Sample 2 | 813 | 835 | 829 | 846 | 831 | 49.03 | 5.9% |
| Sample 3 | 1749 | 1876 | 1816 | 1721 | 1791 | 110.98 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2125-CAP|96-well plate precoated with anti- rat BMPER antibody|1
EK2125-ST|Lyophilized recombinant rat BMPER standard|10ng/tube×2
EK2125-DA|Biotinylated anti- rat BMPER antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
