Description
Boster’s Equine Endothelin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endothelin has been precoated onto 96-well plates. Standards(human peptide, C53-W73) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Endothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine Endothelin amount of sample captured in plate.
Synonyms: Endothelin-1;Preproendothelin-1;PPET1;Endothelin-1;ET-1;Big endothelin-1;EDN1;
Species: Equine EDN1
Assay: 3.9pg/ml-250pg/ml
Sensitivy: <0.5pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from rat|monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: horse equine peptide; Immunogen sequence: C53-W73
Gene name: Endothelin-1 / Endothelin-1
Protein name: Endothelin-1
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 26.1 | 85.4 | 146 | 23.2 | 84.3 | 143 |
| Standard deviation | 1.41 | 3.76 | 8.03 | 1.79 | 6.07 | 8.72 |
| CV(%) | 4.4 | 4.4 | 5.5 | 7.7 | 7.2 | 6.1 |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 11 | 13 | 12 | 14 | 12 | 1.11 | 9.2% |
| Sample 2 | 51 | 49 | 47 | 47 | 48 | 1.65 | 3.4% |
| Sample 3 | 131 | 117 | 110 | 124 | 120 | 7.82 | 6.5% |
*number of samples for each test n=16.
1ng/tube
