Description
Boster’s human HTRA2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HTRA2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A134-E458) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HTRA2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Serine protease HTRA2, mitochondrial; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI; HTRA2; OMI; PRSS25
Species: Human HTRA2
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human HTRA2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A134-E458
Gene name: Serine protease HTRA2, mitochondrial / HtrA serine peptidase 2
Protein name: Serine protease HTRA2, mitochondrial
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|78|249|857|81|254|955
Standard deviation|3.20|11.45|45.42|4.46|17.02|71.63
CV(%)|4.1%|4.6%|5.3%|5.5%|6.7%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 78 | 74 | 82 | 80 | 78 | 2.95 | 3.7% |
| Sample 2 | 249 | 237 | 282 | 261 | 257 | 16.61 | 6.4% |
| Sample 3 | 857 | 951 | 913 | 804 | 881 | 55.74 | 6.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1893-CAP|96-well plate precoated with anti- human HTRA2 antibody|1
EK1893-ST|Lyophilized recombinant human HTRA2 standard|10ng/tube×2
EK1893-DA|Biotinylated anti- human HTRA2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
