Description
Boster’s human VWF-A2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for VWF-A2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: D1498-V1665) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for VWF-A2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF
Species: Human VWF-A2
Assay: 312pg/ml-20000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human VWF-A2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from sheep|a biotinylated detection polyclonal antibody from sheep
Anticoagulant: heparin or EDTA
Immunogen: E.coli, D1498-V1665
Gene name: von Willebrand factor / von Willebrand factor
Protein name: von Willebrand factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|571|1728|9762|600|1987|9834
Standard deviation|35.4|73.3|497.86|40.2|147.04|717.9
CV(%)|6.2%|4.3%|5.1%|6.7%|7.4%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 571 | 552 | 614 | 586 | 580 | 22.66 | 3.9% |
| Sample 2 | 1728 | 1659 | 1612 | 1716 | 1678 | 46.52 | 2.7% |
| Sample 3 | 9762 | 9879 | 8855 | 8135 | 9157 | 711.16 | 7.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1743-CAP|96-well plate precoated with anti- human VWF-A2 antibody|1
EK1743-ST|Lyophilized recombinant human VWF-A2 standard|20ng/tube×2
EK1743-DA|Biotinylated anti- human VWF-A2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
