Description
Boster’s human Klotho ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Klotho has been precoated onto 96-well plates. Standards(NSO,E34-S981) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Klotho is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Klotho amount of sample captured in plate.
Synonyms: Klotho; Klotho peptide; KL
Species: Human KL
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Klotho
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,E34-S981
Gene name: Klotho / klotho
Protein name: Klotho
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|308|1224|5567|337|1220|5449
Standard deviation|15.4|79.56|239.38|20.55|102.48|321.49
CV(%)|5%|6.5%|4.3%|6.1%|8.4%|5.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 308 | 313 | 332 | 326 | 319 | 9.65 | 3% |
| Sample 2 | 1224 | 1059 | 1107 | 1175 | 1141 | 63.09 | 5.5% |
| Sample 3 | 5567 | 6048 | 6003 | 5069 | 5671 | 395.46 | 6.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1688-CAP|96-well plate precoated with anti- human Klotho antibody|1
EK1688-ST|Lyophilized recombinant human Klotho standard|10ng/tubex2
EK1688-DA|Biotinylated anti- human Klotho antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
