Description
Boster’s human ITIH4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ITIH4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E29-L930) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ITIH4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP; Plasma kallikrein sensitive glycoprotein 120; Gp120; PK-120; ITIH4; IHRP; ITIHL1; PK120; PRO1851
Species: Human ITIH4
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human ITIH4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E29-L930
Gene name: Inter-alpha-trypsin inhibitor heavy chain H4 / inter-alpha-trypsin inhibitor heavy chain family member 4
Protein name: Inter-alpha-trypsin inhibitor heavy chain H4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|48|216|746|58|243|852
Standard deviation|1.97|9.72|42.5|3.6|17.7|64.75
CV(%)|4.1%|4.5%|5.7%|6.2%|7.3%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 48 | 49 | 52 | 45 | 48 | 2.50 | 5.2% |
| Sample 2 | 216 | 216 | 209 | 205 | 211 | 4.71 | 2.2% |
| Sample 3 | 746 | 856 | 880 | 835 | 829 | 50.63 | 6.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1670-CAP|96-well plate precoated with anti- human ITIH4 antibody|1
EK1670-ST|Lyophilized recombinant human ITIH4 standard|2ng/tube×2
EK1670-DA|Biotinylated anti- human ITIH4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
