Description
Boster’s rat Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A2-E135） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Galectin-1 amount of sample captured in plate.
Synonyms: Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; RL 14.5; S-Lac lectin 1; Lgals1
Species: Rat Galectin-1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat Galectin-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,A2-E135
Gene name: Galectin-1 / galectin 1
Protein name: Galectin-1
Storage: Store at 4˚C for 6 months, at -20˚C for 15 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|243|1504|5437|262|1512|5561
Standard deviation|15.31|85.71|260.98|17.29|110.4|428.2
CV(%)|6.3%|5.7%|4.8%|6.6%|7.3%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 243 | 248 | 224 | 249 | 241 | 10.07 | 4.1% |
| Sample . 2 |
1504 | 1287 | 1331 | 1330 | 1363 | 83.32 | 6.1%% |
| Sample 3 | 5437 | 6005 | 5471 | 5982 | 5723 | 270.14 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1588-CAP|96-well plate precoated with anti- rat Galectin-1 antibody|1
EK1588-ST|Lyophilized recombinant rat Galectin-1 standard|10ng/tubex2
EK1588-DA|Biotinylated anti- rat Galectin-1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
