Description
Boster’s mouse CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL25 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: Q24-N144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL25 amount of sample captured in plate.
Synonyms: CCL25 | Chemokine TECK | O15444 | SCY A25 | SCYA25 | SCYA25 | TECK | Small inducible cytokine A25 | Small-inducible cytokine A25 | TECKvar | Thymus-expressed chemokine
Species: mouse CCL25
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CCL25
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,Q24-N144
Gene name: C-C motif chemokine 25 / chemokine (C-C motif) ligand 25
Protein name: C-C motif chemokine 25
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|90|258|916|98|271|971
Standard deviation|5.13|17.54|71.44|6.07|20.86|75.73
CV(%)|5.7%|6.8%|7.8%|6.2%|7.7%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 92 | 86 | 94 | 90 | 2.95 | 3.2% |
| Sample 2 | 258 | 283 | 257 | 304 | 275 | 19.47 | 7% |
| Sample 3 | 916 | 985 | 981 | 951 | 958 | 27.7 | 2.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1424-CAP|96-well plate precoated with anti- mouse CCL25 antibody|1
EK1424-ST|Lyophilized recombinant mouse CCL25 standard|10ng/tube×2
EK1424-DA|Biotinylated anti- mouse CCL25 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
