Description
Boster’s human IGF2R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF2R has been precoated onto 96-well plates. Standards(NSO, S1510-F2108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF2R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF2R amount of sample captured in plate.
Synonyms: 300 kDa mannose 6 phosphate receptor|300 kDa mannose 6-phosphate receptor|Cation independent mannose 6 phosphate receptor|Cation-independent mannose-6-phosphate receptor|CD222|CD222 antigen|CI Man 6 P receptor|CI Man-6-P receptor|CI MPR|CI-M6PR|CI-MPR|CIMPR|IGF 2 receptor|IGF 2R|IGF II receptor|IGF-II receptor|IGF2 receptor|IGF2R|Insulin like growth factor 2 receptor|Insulin like growth factor II receptor|Insulin-like growth factor 2 receptor|Insulin-like growth factor II receptor|M6P R|M6P/IGF2 receptor|M6P/IGF2R|M6PR|mannose 6 phosphate receptor|mannose 6 phosphate receptor, cation independent|MPR 300|MPR300|MPRI|MPRI_HUMAN
Species: human IGF2R
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IGF2R
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S1510-F2108
Gene name: Cation-independent mannose-6-phosphate receptor / insulin-like growth factor 2 receptor
Protein name: Cation-independent mannose-6-phosphate receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|249|1351|4462|248|1314|4363
Standard deviation|9.96|77|196.32|13.64|95.92|253.05
CV(%)|4%|5.7%|4.4%|5.5%|7.3%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 249 | 245 | 232 | 224 | 237 | 10.01 | 4.2% |
| Sample 2 | 1351 | 1344 | 1406 | 1445 | 1386 | 41.43 | 2.9% |
| Sample 3 | 4462 | 4544 | 5035 | 4402 | 4610 | 250.07 | 5.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1326-CAP|96-well plate precoated with anti- human IGF2R antibody|1
EK1326-ST|Lyophilized recombinant human IGF2R standard|10ng/tube×2
EK1326-DA|Biotinylated anti- human IGF2R antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
