Description
Boster’s human Galectin-3BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-3BP has been precoated onto 96-well plates. Standards(NSO,V19-D585) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Galectin-3BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-3BP amount of sample captured in plate.
Synonyms: 90K|90K MAC 2 BP|Basement membrane autoantigen p105|BTBD17B|Galectin 3 binding protein|Galectin-3-binding protein|L3 antigen|Lectin galactoside binding soluble 3 binding protein|Lectin galactoside-binding soluble 3-binding protein|LG3BP_HUMAN|LGALS3BP|M2BP|Mac 2 binding protein|Mac 2 BP|Mac-2 BP|Mac-2-binding protein|Mac2 BP|MAC2BP|Serum protein 90K|TANGO10B|Transport and golgi organization 10 homolog B|Tumor associated antigen 90K|Tumor-associated antigen 90K
Species: human Galectin-3BP
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Galectin-3BP
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse
Anticoagulant: heparin
Immunogen: NSO, V19-D585
Gene name: Galectin-3-binding protein / lectin, galactoside-binding, soluble, 3 binding protein
Protein name: Galectin-3-binding protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|312|1090|4158|326|1154|3768
Standard deviation|18.72|63.22|282.74|20.86|84.24|271.29
CV(%)|6%|5.8%|6.8%|6.4%|7.3%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 312 | 367 | 361 | 366 | 351 | 22.91 | 6.5% |
| Sample 2 | 1090 | 1067 | 1143 | 1041 | 1085 | 37.57 | 3.4% |
| Sample 3 | 4158 | 3836 | 4350 | 4159 | 4125 | 184.65 | 4.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1240-CAP|96-well plate precoated with anti- human Galectin-3BP antibody|1
EK1240-ST|Lyophilized recombinant human Galectin-3BP standard|10ng/tube×2
EK1240-DA|Biotinylated anti- human Galectin-3BP antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
