Description
Boster’s human CYR61 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CYR61 has been precoated onto 96-well plates. Standards(CHO, A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYR61 amount of sample captured in plate.
Synonyms: 3CH61|CCN 1|CCN family member 1|CCN1|CCN1|CYR 61|Cyr61|CYR61 protein|CYR61 protein precursor|CYR61 protein precursor|CYR61_HUMAN|Cysteine rich angiogenic inducer 61|Cysteine rich heparin binding protein 61|Cysteine-rich angiogenic inducer 61|GIG 1|GIG1|GIG1 protein|IBP-10|IGF-binding protein 10|Igfbp 10|IGFBP-10|IGFBP10|Insulin like growth factor binding protein 10|Insulin-like growth factor-binding protein 10|Protein CYR61|Protein GIG1
Species: human CYR61
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CYR61
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: CHO, A22-D381
Gene name: Protein CYR61 / cysteine-rich, angiogenic inducer, 61
Protein name: Protein CYR61
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|61|248|814|59|242|806
Standard deviation|2.86|16.12|55.35|3.3|17.66|64.48
CV(%)|4.7%|6.5%|6.8%|5.6%|7.3%|8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 61 | 64 | 63 | 58 | 61 | 2.29 | 3.7% |
| Sample 2 | 248 | 273 | 263 | 255 | 259 | 9.31 | 3.5% |
| Sample 3 | 814 | 907 | 783 | 869 | 843 | 47.99 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1203-CAP|96-well plate precoated with anti- human CYR61 antibody|1
EK1203-ST|Lyophilized recombinant human CYR61 standard|10ng/tube×2
EK1203-DA|Biotinylated anti- human CYR61 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
