Description
Boster’s human CCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL14 has been precoated onto 96-well plates. Standards(E.coli, T20-N93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL14 amount of sample captured in plate.
Synonyms: C-C motif chemokine 14|CC-1|CC-3|Chemokine (C-C motif) ligand 14|Chemokine CC-1/CC-3|Chemokine CC-3|CKB1|HCC-1|HCC-1(1-74)|HCC-1/HCC-3|HCC-3|MCIF|NCC-2|NCC2|New CC chemokine 2|SCYA14|SCYL2|Small inducible cytokine subfamily A (Cys-Cys), member 14|Small-inducible cytokine A14|SY14
Species: human CCL14
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL14
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, T20-N93
Gene name: C-C motif chemokine 14 / chemokine (C-C motif) ligand 14
Protein name: C-C motif chemokine 14
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|37|238|445|40|227|446
Standard deviation|1.92|13.8|25.81|2.08|15.89|33.45
CV(%)|5.2%|5.8%|5.8%|5.2%|7%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 37 | 34 | 36 | 35 | 35 | 1.11 | 3.1% |
| Sample 2 | 238 | 250 | 251 | 216 | 238 | 14.09 | 5.9% |
| Sample 3 | 445 | 501 | 523 | 499 | 492 | 28.72 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1123-CAP|96-well plate precoated with anti- human CCL14 antibody|1
EK1123-ST|Lyophilized recombinant human CCL14 standard|10ng/tube×2
EK1123-DA|Biotinylated anti- human CCL14 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
