Description
Boster’s human CCL15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL15 has been precoated onto 96-well plates. Standards(E.coli, Q22-I113) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL15 amount of sample captured in plate.
Synonyms: CC chemokine 3|_x000D_CCL15|_x000D_CCL15(29-92)|_x000D__x000D__x000D_CCL15_HUMAN|_x000D_Chemokine CC-2|_x000D_Chemokine CC2|_x000D_HCC-2|_x000D_HCC2|_x000D_HMRP 2B|_x000D_HMRP2B|_x000D_Leukotactin 1|_x000D_Leukotactin-1|_x000D_LKN-1|_x000D_LKN1|_x000D_Macrophage inflammatory protein 5|_x000D_MIP-1 delta|_x000D_MIP-5|_x000D_MIP1 delta|_x000D_MIP1d|_x000D_MIP5|_x000D_Mrp-2b|_x000D_Mrp2b|_x000D_NCC-3|_x000D_NCC3|_x000D_SCYA15|_x000D_SCYL3|_x000D_Small inducible cytokine A15|_x000D_Small inducible cytokine subfamily A (Cys-Cys), member 15|_x000D_Small-inducible cytokine A15|_x000D_SY15
Species: human CCL15
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL15
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, Q22-I113
Gene name: C-C motif chemokine 15 / chemokine (C-C motif) ligand 15
Protein name: C-C motif chemokine 15
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|33|185|439|31|188|417
Standard deviation|2.31|9.8|31.6|2.75|13.34|37.11
CV(%)|7%|5.3%|7.2%|8.9%|7.1%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 29 | 29 | 33 | 31 | 2 | 6.4% |
| Sample 2 | 185 | 185 | 181 | 184 | 183 | 1.63 | 0.8% |
| Sample 3 | 439 | 430 | 478 | 439 | 446 | 18.55 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1120-CAP|96-well plate precoated with anti- human CCL15 antibody|1
EK1120-ST|Lyophilized recombinant human CCL15 standard|10ng/tube×2
EK1120-DA|Biotinylated anti- human CCL15 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
