Description
Boster’s human A2M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for A2M has been precoated onto 96-well plates. Standards(from human plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for A2M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human A2M amount of sample captured in plate.
Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|A2m|A2MD|A2MG_HUMAN|Alpha-2-M|Alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|CPAMD5|FWP007|Pregnancy zone protein|S863-7
Species: human A2M
Assay: 625pg/ml-40000pg/ml
Sensitivy: <20pg/ml Specifity: Natural human A2M
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: from human plasma
Gene name: Alpha-2-macroglobulin / alpha-2-macroglobulin
Protein name: Alpha-2-macroglobulin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1367|3876|26784|1316|3942|26889
Standard deviation|97.05|236.43|2062.36|103.96|240.46|2151.12
CV(%)|7.1%|6.1%|7.7%|7.9%|6.1%|8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1367 | 1285 | 1469 | 1391 | 1378 | 65.61 | 4.7% |
| Sample 2 | 3876 | 3671 | 3260 | 3321 | 3532 | 253.06 | 7.1% |
| Sample 3 | 26784 | 32034 | 29630 | 29138 | 29396 | 1864.33 | 6.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1118-CAP|96-well plate precoated with anti- human A2M antibody|1
EK1118-ST|Lyophilized recombinant human A2M standard|40ng/tube×2
EK1118-DA|Biotinylated anti- human A2M antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
