Description
Boster’s human MIA ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIA has been precoated onto 96-well plates. Standards(E.coli, G25-131Q) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIA amount of sample captured in plate.
Synonyms: CD RAP|_x000D_melanoma inhibitory activity|_x000D_Melanoma inhibitory activity protein|_x000D__x000D__x000D_Melanoma-derived growth regulatory protein|_x000D_Mia|_x000D_MIA_HUMAN
Species: Human MIA
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MIA
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, G25-131Q
Gene name: Melanoma-derived growth regulatory protein / melanoma inhibitory activity
Protein name: Melanoma-derived growth regulatory protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|29|172|485|26|165|509
Standard deviation|1.91|13.41|32.98|1.97|15.84|35.12
CV(%)|6.6%|7.8%|6.8%|7.6%|9.6%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 28 | 27 | 29 | 28 | 0.82 | 2.9% |
| Sample 2 | 172 | 166 | 171 | 170 | 169 | 2.27 | 1.3% |
| Sample 3 | 485 | 453 | 511 | 458 | 476 | 23.22 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0941-CAP|96-well plate precoated with anti- human MIA antibody|1
EK0941-ST|Lyophilized recombinant human MIA standard|1ng/tube×2
EK0941-DA|Biotinylated anti- human MIA antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
