Description
Boster’s human ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM12 amount of sample captured in plate. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
Synonyms: ADA12_HUMAN|_x000D_ADAM 12|_x000D_ADAM metallopeptidase domain 12|_x000D__x000D__x000D_ADAM12|_x000D_Disintegrin and metalloproteinase domain-containing protein 12|_x000D_MCMP|_x000D_MCMPMltna|_x000D_Meltrin-alpha|_x000D_MLTN|_x000D_MLTNA
Species: Human ADAM12
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human ADAM12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: CHO, R29-S513
Gene name: Disintegrin and metalloproteinase domain-containing protein 12 / ADAM metallopeptidase domain 12
Protein name: Disintegrin and metalloproteinase domain-containing protein 12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|285|1490|4755|259|1498|4763
Standard deviation|16.81|75.99|256.77|19.68|98.86|338.17
CV(%)|5.9%|5.1%|5.4%|7.6%|6.6%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 285 | 300 | 321 | 287 | 298 | 14.34 | 4.8% |
| Sample 2 | 1490 | 1376 | 1529 | 1374 | 1442 | 68.65 | 4.7% |
| Sample 3 | 4755 | 4542 | 4639 | 5146 | 4770 | 229.53 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0934-CAP|96-well plate precoated with anti- human ADAM12 antibody|1
EK0934-ST|Lyophilized recombinant human ADAM12 standard|10ng/tube×2
EK0934-DA|Biotinylated anti- human ADAM12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
