Description
Boster’s human TNFSF13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFSF13 has been precoated onto 96-well plates. Standards (NSO,A105-L250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFSF13 amount of sample captured in plate.
Synonyms: A proliferation-inducing ligand|APRIL|CD256|TALL-2|TALL2|TNF- and APOL-related leukocyte expressed ligand 2|TNF-related death ligand 1|TNF13_HUMAN|TNFSF13|TRDL-1|Tumor necrosis factor (ligand) superfamily member 13|Tumor necrosis factor ligand superfamily member 13|Tumor necrosis factor like protein ZTNF2|Tumor necrosis factor related death ligand 1|ZTNF2
Species: Human TNFSF13
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TNFSF13
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,A105-L250
Gene name: Tumor necrosis factor ligand superfamily member 13 / tumor necrosis factor (ligand) superfamily, member 13
Protein name: Tumor necrosis factor ligand superfamily member 13
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|264|1700|4235|241|1784|4408
Standard deviation|14.25|119|237.16|15.42|144.5|317.37
CV(%)|5.4%|7%|5.6%|6.4%|8.1%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 264 | 265 | 272 | 285 | 271 | 8.38 | 3% |
| Sample 2 | 1700 | 1548 | 1563 | 1481 | 1573 | 79.55 | 5% |
| Sample 3 | 4235 | 4377 | 4466 | 4871 | 4487 | 236.37 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0921-CAP|96-well plate precoated with anti- human TNFSF13 antibody|1
EK0921-ST|Lyophilized recombinant human TNFSF13 standard|10ng/tube×2
EK0921-DA|Biotinylated anti- human TNFSF13 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
