Description
Boster’s human Furin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Furin has been precoated onto 96-well plates. Standards(NSO, D108-E715) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Furin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Furin amount of sample captured in plate.
Synonyms: Dibasic processing enzyme|Dibasic-processing enzyme|FES upstream region|FUR|FURIN|Furin membrane associated receptor protein|FURIN_HUMAN|PACE|Paired basic amino acid residue cleaving enzyme|Paired basic amino acid residue-cleaving enzyme|PCSK3|Proprotein convertase subtilisin/kexin type 3|SPC1
Species: Human Furin
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Furin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, D108-E715
Gene name: Furin / furin (paired basic amino acid cleaving enzyme)
Protein name: Furin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|417|2751|8293|457|2701|7870
Standard deviation|21.68|143.05|464.4|28.79|148.55|448.59
CV(%)|5.2%|5.2%|5.6%|6.3%|5.5%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 417 | 420 | 397 | 384 | 404 | 14.77 | 3.6% |
| Sample 2 | 2751 | 2551 | 2738 | 2985 | 2756 | 153.96 | 5.5% |
| Sample 3 | 8293 | 8993 | 7686 | 9246 | 8554 | 610.96 | 7.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0916-CAP|96-well plate precoated with anti- human Furin antibody|1
EK0916-ST|Lyophilized recombinant human Furin standard|20ng/tube×2
EK0916-DA|Biotinylated anti- human Furin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
