Description
Boster’s human CD23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD23 has been precoated onto 96-well plates. Standards(NSO, D48-S321) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD23 amount of sample captured in plate.
Synonyms: BLAST 2|BLAST-2|C-type lectin domain family 4 member J|CD23|CD23 antigen|CD23A|CLEC4J|Fc fragment of IgE low affinity II receptor for (CD23)|Fc-epsilon-RII|FCE2|Fcer2|FCER2_HUMAN|IGEBF|Immunoglobulin E-binding factor|Low affinity immunoglobulin epsilon Fc receptor|Low affinity immunoglobulin epsilon Fc receptor soluble form|Lymphocyte IgE receptor
Species: Human CD23
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD23
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, D48-S321
Gene name: Low affinity immunoglobulin epsilon Fc receptor / Fc fragment of IgE, low affinity II, receptor for (CD23)
Protein name: Low affinity immunoglobulin epsilon Fc receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|61|194|981|55|207|899
Standard deviation|2.8|7.95|64.74|3.41|9.93|71.02
CV(%)|4.6%|4.1%|6.6%|6.2%|4.8%|7.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 61 | 71 | 62 | 62 | 64 | 4.06 | 6.3% |
| Sample 2 | 194 | 218 | 229 | 235 | 219 | 15.66 | 7.1% |
| Sample 3 | 981 | 870 | 950 | 851 | 913 | 54.05 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0912-CAP|96-well plate precoated with anti- human CD23 antibody|1
EK0912-ST|Lyophilized recombinant human CD23 standard|10ng/tube×2
EK0912-DA|Biotinylated anti- human CD23 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
