Description
Boster’s human HSP27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HSP27 has been precoated onto 96-well plates. Standards(E.coli, T2-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HSP27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HSP27 amount of sample captured in plate.
Synonyms: HSPB1|_x000D_28 kDa heat shock protein|_x000D_CMT2F|_x000D__x000D__x000D_estrogen regulated 24 kDa protein|_x000D_Estrogen-regulated 24 kDa protein|_x000D_Heat shock 27 kDa protein|_x000D_heat shock 27kDa protein 1|_x000D_heat shock protein beta 1|_x000D_Heat shock protein beta-1|_x000D_HMN2B|_x000D_HS.76067|_x000D_HSP 27|_x000D_Hsp25|_x000D_HSP27|_x000D_HSP28|_x000D_HspB1|_x000D_SRP27|_x000D_stress responsive protein 27|_x000D_Stress-responsive protein 27
Species: Human HSP27
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human HSP27
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, T2-K205
Gene name: Heat shock protein beta-1 / heat shock 27kDa protein 1
Protein name: Heat shock protein beta-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|82|492|1757|81|468|1729
Standard deviation|3.44|26.07|110.69|4.53|33.69|121.03
CV(%)|4.2%|5.3%|6.3%|5.6%|7.2%|7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 88 | 78 | 91 | 84 | 5.06 | 6% |
| Sample 2 | 492 | 526 | 485 | 501 | 501 | 15.5 | 3% |
| Sample 3 | 1757 | 1541 | 1607 | 1549 | 1613 | 86.67 | 5.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0881-CAP|96-well plate precoated with anti- human HSP27 antibody|1
EK0881-ST|Lyophilized recombinant human HSP27 standard|10ng/tube×2
EK0881-DA|Biotinylated anti- human HSP27 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
