Description
Boster’s mouse RAGE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RAGE has been precoated onto 96-well plates. Standards(NSO, Q24-A342) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RAGE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RAGE amount of sample captured in plate.
Synonyms: Advanced glycosylation end product specific receptor|_x000D_Advanced glycosylation end product-specific receptor|_x000D_Ager|_x000D__x000D__x000D_DAMA 358M23.4|_x000D_LE 9211 A antigen |_x000D_MAPK MAK MRK overlapping kinase |_x000D_MGC22357|_x000D_MOK|_x000D_OTTHUMP00000174885|_x000D_RAGE 1|_x000D_RAGE isoform NtRAGE-delta|
RAGE isoform sRAGE-delta|RAGE_HUMAN|_x000D_RAGE1|_x000D_Receptor for advanced glycation end products|_x000D_receptor for advanced glycation end-products variant 20|Receptor for advanced glycosylation end products|_x000D_Renal tumor antigen |_x000D_Renal tumor antigen 1
Species: Mouse RAGE
Assay: 78pg/ml-5000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse RAGE
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q24-A342
Gene name: Advanced glycosylation end product-specific receptor / advanced glycosylation end product-specific receptor
Protein name: Advanced glycosylation end product-specific receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|113|797|2158|103|830|2180
Standard deviation|4.63|55.79|90.63|5.15|61.42|130.8
CV(%)|4.1%|7%|4.2%|5%|7.4%|6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 113 | 119 | 116 | 113 | 115 | 2.48 | 2.1% |
| Sample 2 | 797 | 796 | 771 | 805 | 792 | 12.75 | 1.6% |
| Sample 3 | 2158 | 2179 | 2112 | 2263 | 2178 | 54.73 | 2.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0828-CAP|96-well plate precoated with anti- mouse RAGE antibody|1
EK0828-ST|Lyophilized recombinant mouse RAGE standard|10ng/tube×2
EK0828-DA|Biotinylated anti- mouse RAGE antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
