Description
Boster’s mouse IL-27 p28 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-27 p28 has been precoated onto 96-well plates. Standards(NSO, F29-S234) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-27 p28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-27 p28 amount of sample captured in plate.
Synonyms: IL 27A|_x000D_IL 30|_x000D_IL-27 subunit alpha|_x000D__x000D__x000D_IL-27-A|_x000D_IL27|_x000D_IL27-A|_x000D_IL27A|_x000D_IL27A_HUMAN|_x000D_IL27p28|_x000D_IL30|_x000D_Interleukin 27|_x000D_Interleukin 27 subunit alpha|_x000D_Interleukin 30|_x000D_Interleukin-27 subunit alpha|_x000D_p28
Species: Mouse IL-27 p28
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse IL-27 p28
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, F29-S234
Gene name: Interleukin-27 subunit alpha / interleukin 27
Protein name: Interleukin-27 subunit alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|35|197|420|31|207|394
Standard deviation|1.68|9.65|27.3|1.61|12.83|25.61
CV(%)|4.8%|4.9%|6.5%|5.2%|6.2%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 41 | 39 | 37 | 38 | 2.23 | 5.8% |
| Sample 2 | 197 | 206 | 189 | 190 | 195 | 6.8 | 3.4% |
| Sample 3 | 420 | 442 | 424 | 441 | 431 | 9.85 | 2.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0800-CAP|96-well plate precoated with anti- mouse IL-27 p28 antibody|1
EK0800-ST|Lyophilized recombinant mouse IL-27 p28 standard|1ng/tube×2
EK0800-DA|Biotinylated anti- mouse IL-27 p28 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
