Description
Boster’s human ErbB-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for ErbB-2 has been precoated onto 96-well plates. Standards(NSO, T23-T652) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ErbB-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ErbB-2 amount of sample captured in plate.
Synonyms: c erb B2|C erbB 2|CD340|CD340 antigen|Cerb B2/neu protein|CerbB2|Erb B2|erbb2|HER 2|HER 2/neu|HER2|Her2/neu|Herstatin|Metastatic lymph node gene 19 protein|MLN 19|MLN19|NEU|NEU Proto Oncogene|Neuro Glioblastoma Derived Oncogene Homolog|Neuroblastoma/glioblastoma derived oncogene homolog|NGL|p185 ErbB2|p185erbB2|Proto oncogene c ErbB 2|Proto oncogene Neu|Receptor protein tyrosine kinase ErbB2 precursor|Receptor tyrosine protein kinase erbB 2|TKR1|Tyrosine kinase type cell surface receptor HER2|v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian)|Verb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog
Species: Human ErbB-2
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human ErbB-2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, T23-T652
Gene name: Receptor tyrosine-protein kinase erbB-2 / v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
Protein name: Receptor tyrosine-protein kinase erbB-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|164|544|1478|167|559|1477
Standard deviation|12.79|38.62|93.11|16.03|40.8|104.86
CV(%)|7.8%|7.1%|6.3%|9.6%|7.3%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 164 | 178 | 168 | 153 | 165 | 8.95 | 5.4% |
| Sample 2 | 544 | 528 | 493 | 539 | 526 | 19.91 | 3.7% |
| Sample 3 | 1478 | 1506 | 1587 | 1640 | 1552 | 64.34 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0756-CAP|96-well plate precoated with anti- human ErbB-2 antibody|1
EK0756-ST|Lyophilized recombinant human ErbB-2 standard|10ng/tube×2
EK0756-DA|Biotinylated anti- human ErbB-2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
