Description
Boster’s human CXCL11 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL11 has been precoated onto 96-well plates. Standards(E.coli, F22-F94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL11 amount of sample captured in plate.
Synonyms: b R1|Beta R1|Beta-R1|betaR1|C X C motif chemokine 11|C-X-C motif chemokine 11|Chemokine (C-X-C motif) ligand 11|Chemokine C-X-C motif ligand 11|CXCL11|CXL11_HUMAN|H174|I TAC|I-TAC|Interferon gamma inducible protein 9|Interferon gamma-inducible protein 9|Interferon inducible T cell a chemoattractant I TAC|Interferon inducible T cell alpha chemoattractant|Interferon-inducible T-cell alpha chemoattractant|IP 9|IP-9|IP9|ITAC|MGC102770|SCYB11|SCYB9B|Small inducible cytokine B11|Small inducible cytokine subfamily B (Cys X Cys) member 11|Small inducible cytokine subfamily B (Cys X Cys) member 9B|Small inducible cytokine subfamily B, member 11|Small inducible cytokine subfamily B, member 9B|Small-inducible cytokine B11
Species: Human CXCL11
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CXCL11
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, F22-F94
Gene name: C-X-C motif chemokine 11 / chemokine (C-X-C motif) ligand 11
Protein name: C-X-C motif chemokine 11
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|107|712|1603|100|764|1565
Standard deviation|7.91|47.7|83.35|8.7|64.94|82.94
CV(%)|7.4%|6.7%|5.2%|8.7%|8.5%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 107 | 122 | 102 | 116 | 111 | 7.75 | 6.9% |
| Sample 2 | 712 | 667 | 661 | 672 | 678 | 20.01 | 2.9% |
| Sample 3 | 1603 | 1507 | 1537 | 1543 | 1547 | 34.82 | 2.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0737-CAP|96-well plate precoated with anti- human CXCL11 antibody|1
EK0737-ST|Lyophilized recombinant human CXCL11 standard|10ng/tube×2
EK0737-DA|Biotinylated anti- human CXCL11 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
