Description
Boster’s human CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, A37-N114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL5 amount of sample captured in plate.
Synonyms: AMCF II|AMCFII|C X C motif chemokine 5|Chemokine (C X C motif) ligand 5|CXCL 5|ENA 78|ENA78|Epithelial derived neutrophil activating protein 78|GCP 2|LIX|Neutrophil activating peptide ENA 78|Neutrophil activating protein 78|SCYB5|Scyb6|Small inducible cytokine B5|Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78)|Small inducible cytokine subfamily B, member 15
Species: Human CXCL5
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CXCL5
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A37-N114
Gene name: C-X-C motif chemokine 5 / chemokine (C-X-C motif) ligand 5
Protein name: C-X-C motif chemokine 5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|66|270|964|61|293|970
Standard deviation|2.77|18.09|74.22|3.53|22.26|82.45
CV(%)|4.2%|6.7%|7.7%|5.8%|7.6%|8.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 66 | 77 | 65 | 68 | 69 | 4.74 | 6.8% |
| Sample 2 | 270 | 257 | 287 | 288 | 275 | 12.85 | 4.6% |
| Sample 3 | 964 | 912 | 900 | 813 | 897 | 54.26 | 6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0728-CAP|96-well plate precoated with anti- human CXCL5 antibody|1
EK0728-ST|Lyophilized recombinant human CXCL5 standard|10ng/tube×2
EK0728-DA|Biotinylated anti- human CXCL5 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
