Description
Boster’s mouse CXCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL4 has been precoated onto 96-well plates. Standards(E.coli, V30-S105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL4 amount of sample captured in plate.
Synonyms: C X C motif chemokine 4|C-X-C motif chemokine 4|Chemokine (C X C motif) ligand 4|Chemokine (CXC motif) ligand 4|Chemokine, CXC motif, ligand 4|CXCL 4|CXCL4|Iroplact|MGC138298|Oncostatin A|Oncostatin-A|OncostatinA|PF 4|PF-4|PF4|Pf4a|Platelet factor 4|PLF4_HUMAN|RATPF4A|SCYB 4|SCYB4|short form|Small inducible cytokine subfamily B, member 4|Small inducible cytokine subfamily member 4
Species: Mouse CXCL4
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant mouse CXCL4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, V30-S105
Gene name: Platelet factor 4 / platelet factor 4
Protein name: Platelet factor 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|32|263|425|33|253|425
Standard deviation|1.69|11.04|28.05|2.27|15.18|30.6
CV(%)|5.3%|4.2%|6.6%|6.9%|6%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 32 | 34 | 33 | 32 | 0.82 | 2.5% |
| Sample 2 | 263 | 259 | 241 | 228 | 247 | 14.09 | 5.7% |
| Sample 3 | 425 | 469 | 430 | 397 | 430 | 25.66 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0727-CAP|96-well plate precoated with anti- mouse CXCL4 antibody|1
EK0727-ST|Lyophilized recombinant mouse CXCL4 standard|10ng/tube×2
EK0727-DA|Biotinylated anti- mouse CXCL4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
