Description
Boster’s human IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL7R has been precoated onto 96-well plates. Standards(NSO, E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL7R amount of sample captured in plate.
Synonyms: CD 127|CD127|CD127 antigen|CDw127|IL 7R alpha|IL 7R|IL-7 receptor subunit alpha|IL-7R subunit alpha|IL-7R-alpha|IL-7RA|IL7R|IL7RA|IL7RA_HUMAN|IL7Ralpha|ILRA|Interleukin 7 receptor alpha chain|Interleukin 7 receptor|Interleukin 7 receptor isoform H5 6|Interleukin-7 receptor subunit alpha
Species: Human IL7R
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human IL7R
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, E21-I262
Gene name: Interleukin-7 receptor subunit alpha / interleukin 7 receptor
Protein name: Interleukin-7 receptor subunit alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|410|2617|9061|443|2807|9233
Standard deviation|25.42|120.38|516.47|33.22|140.35|627.84
CV(%)|6.2%|4.6%|5.7%|7.5%|5%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 410 | 395 | 383 | 426 | 403 | 16.13 | 4% |
| Sample 2 | 2617 | 2389 | 2451 | 2237 | 2423 | 136.17 | 5.6% |
| Sample 3 | 9061 | 9810 | 8729 | 10576 | 9544 | 712.96 | 7.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0713-CAP|96-well plate precoated with anti- human IL7R antibody|1
EK0713-ST|Lyophilized recombinant human IL7R standard|20ng/tube×2
EK0713-DA|Biotinylated anti- human IL7R antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
