Description
Boster’s human CCL18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL18 has been precoated onto 96-well plates. Standards(E.coli, A21-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL18 amount of sample captured in plate.
Synonyms: Alternative macrophage activation associated CC chemokine 1|Alternative macrophage activation-associated CC chemokine 1|AMAC 1|AMAC-1|AMAC1|C C motif chemokine 18|CC chemokine ligand 18|CC chemokine PARC|CCL18|CCL18(4-69)|CCL18_HUMAN|Chemokine (C C motif) ligand 18|Chemokine (C C) dendritic|Chemokine ligand 18|CKb7|DC CK1|DC-CK1|DCCK1|Dendritic cell chemokine 1|Macrophage inflammatory protein 4|MIP 4|MIP-4|MIP4|PARC|Pulmonary and activation regulated chemokine|Pulmonary and activation-regulated chemokine|Small inducible cytokine A18|Small-inducible cytokine A18
Species: Human CCL18
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL18
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli, A21-A89
Gene name: C-C motif chemokine 18 / chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
Protein name: C-C motif chemokine 18
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|18|49|269|18|52|279
Standard deviation|0.99|3.52|20.17|1.29|4.62|22.59
CV(%)|5.5%|7.2%|7.5%|7.2%|8.9%|8.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 18 | 17 | 18 | 18 | 17 | 0.43 | 2.5% |
| Sample 2 | 49 | 56 | 49 | 48 | 50 | 3.2 | 6.4% |
| Sample 3 | 269 | 295 | 278 | 296 | 284 | 11.45 | 4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0686-CAP|96-well plate precoated with anti- human CCL18 antibody|1
EK0686-ST|Lyophilized recombinant human CCL18 standard|10ng/tubex2
EK0686-DA|Biotinylated anti- human CCL18 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
