Description
Boster’s human E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards(NSO, D155-I707) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human E-Cadherin amount of sample captured in plate.
Synonyms: Arc 1|_x000D_Cadherin 1|_x000D_cadherin 1 type 1 E-cadherin|_x000D__x000D__x000D_Cadherin1|_x000D_CAM 120/80|_x000D_CD 234|_x000D_CD324|_x000D_CD324 antigen|_x000D_CDH1|_x000D_CDHE|_x000D_ECAD|_x000D_Epithelial cadherin|_x000D_epithelial calcium dependant adhesion protein|_x000D_LCAM|_x000D_Liver cell adhesion molecule|_x000D_UVO|_x000D_Uvomorulin
Species: Human E-Cadherin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <15pg/ml Specifity: Natural and recombinant human E-Cadherin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, D155-I707
Gene name: Cadherin-1 / cadherin 1, type 1, E-cadherin (epithelial)
Protein name: Cadherin-1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|265|1264|4789|253|1258|4691
Standard deviation|15.37|58.14|325.65|16.69|61.64|318.98
CV(%)|5.8%|4.6%|6.8%|6.6%|4.9%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 265 | 266 | 300 | 267 | 274 | 14.73 | 5.3% |
| Sample 2 | 1264 | 1074 | 1156 | 1112 | 1151 | 71.13 | 6.1% |
| Sample 3 | 4789 | 4996 | 5212 | 5707 | 5176 | 341.11 | 6.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0561-CAP|96-well plate precoated with anti- human E-Cadherin antibody|1
EK0561-ST|Lyophilized recombinant human E-Cadherin standard|10ng/tubex2
EK0561-DA|Biotinylated anti- human E-Cadherin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
