Description
Boster’s human VCAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VCAM-1 has been precoated onto 96-well plates. Standards (NSO,F25 – E698) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VCAM-1 amount of sample captured in plate.
Synonyms: CD106|_x000D_CD106 antigen|_x000D_DKFZp779G2333|_x000D__x000D__x000D_INCAM 100|_x000D_INCAM-100|_x000D_INCAM100|_x000D_L1CAM|_x000D_MGC99561|_x000D_V CAM 1|_x000D_V-CAM 1|_x000D_Vascular cell adhesion molecule 1|_x000D_Vascular cell adhesion protein 1|_x000D_VCAM 1|_x000D_VCAM-1|_x000D_VCAM1|_x000D_VCAM1_HUMAN
Species: Human VCAM-1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human VCAM-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,F25 – E698
Gene name: Vascular cell adhesion protein 1 / vascular cell adhesion molecule 1
Protein name: Vascular cell adhesion protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|310|1065|5287|304|1029|5603
Standard deviation|17.67|51.12|385.95|19.15|65.85|481.85
CV(%)|5.7%|4.8%|7.3%|6.3%|6.4%|8.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 310 | 273 | 296 | 273 | 288 | 15.79 | 5.4% |
| Sample 2 | 1065 | 1111 | 1014 | 939 | 1032 | 63.84 | 6.1% |
| Sample 3 | 5287 | 5539 | 5884 | 6073 | 5695 | 303.89 | 5.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0537-CAP|96-well plate precoated with anti- human VCAM-1 antibody|1
EK0537-ST|Lyophilized recombinant human VCAM-1 standard|10ng/tubex2
EK0537-DA|Biotinylated anti- human VCAM-1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
