Description
Boster’s human CCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL4 amount of sample captured in plate.
Synonyms: ACT 2|ACT2|AT744.1|C C motif chemokine 4|CCL4|CCL4L1|Chemokine CC Motif Ligand 4|G 26 T lymphocyte-secreted protein|HC21|LAG 1|LAG1|Lymphocyte activation gene 1|Lymphocyte activation gene 1 protein|MGC104418|MGC126025|MGC126026|MIP 1 beta|MIP1B|PAT 744|Protein H400|SCYA2|SCYA4|Secreted protein G 26|SIS gamma|Small inducible cytokine A4|T cell activation protein 2
Species: Human CCL4
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CCL4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A24-N92
Gene name: C-C motif chemokine 4 / chemokine (C-C motif) ligand 4
Protein name: C-C motif chemokine 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|13|90|254|12|95|256
Standard deviation|0.63|4.95|13.2|0.62|6.84|15.87
CV(%)|4.9%|5.5%|5.2%|5.2%|7.2%|6.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 13 | 11 | 13 | 14 | 12 | 1.08 | 9% |
| Sample 2 | 90 | 88 | 90 | 93 | 90 | 1.78 | 1.9% |
| Sample 3 | 254 | 290 | 285 | 250 | 269 | 17.89 | 6.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0450-CAP|96-well plate precoated with anti- human CCL4 antibody|1
EK0450-ST|Lyophilized recombinant human CCL4 standard|10ng/tubex2
EK0450-DA|Biotinylated anti- human CCL4 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
