Description
Boster’s mouse CCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL3 has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL3 amount of sample captured in plate.
Synonyms: C C motif chemokine 3|CCL3|CCL3_HUMAN|Chemokine (C C motif) ligand 3|Chemokine ligand 3|G0/G1 switch regulatory protein 19 1|G0/G1 switch regulatory protein 19-1|G0S19 1|G0S19 1 protein|Heparin binding chemotaxis protein|L2G25B|LD78-alpha(4-69)|LD78ALPHA|Macrophage inflammatory protein 1 alpha|Macrophage inflammatory protein 1-alpha|MIP 1 alpha|MIP-1-alpha|MIP-1-alpha(4-69)|MIP1A|PAT 464.1|SCYA 3|SCYA3|SIS alpha|SIS beta|SIS-beta|Small inducible cytokine A3|Small-inducible cytokine A3|Tonsillar lymphocyte LD78 alpha protein
Species: Mouse CCL3
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CCL3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A24-A92
Gene name: C-C motif chemokine 3 / chemokine (C-C motif) ligand 3
Protein name: C-C motif chemokine 3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|18|74|240|18|69|249
Standard deviation|0.73|3.99|9.6|0.75|4.96|14.44
CV(%)|4.1%|5.4%|4%|4.2%|7.2%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 18 | 19 | 17 | 17 | 17 | 0.82 | 4.8% |
| Sample 2 | 74 | 69 | 76 | 69 | 72 | 3.08 | 4.2% |
| Sample 3 | 240 | 225 | 228 | 207 | 225 | 11.81 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0449-CAP|96-well plate precoated with anti- mouse CCL3 antibody|1
EK0449-ST|Lyophilized recombinant mouse CCL3 standard|10ng/tubex2
EK0449-DA|Biotinylated anti- mouse CCL3 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
