Description
Boster’s human HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HGF has been precoated onto 96-well plates. Standards(sf21, Q32-R494( alpha) & V495-S728( beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HGF amount of sample captured in plate.
Synonyms: DFNB39|_x000D_F TCF|_x000D_Fibroblast derived tumor cytotoxic factor|_x000D__x000D__x000D_Hepapoietin A|_x000D_Hepatocyte growth factor|_x000D_Hepatocyte growth factor (hepapoietin A scatter factor|_x000D_Hepatocyte growth factor beta chain|_x000D_Hepatopoietin-A|_x000D_Hgf|_x000D_HGF_HUMAN|_x000D_HGFB|_x000D_HPTA|_x000D_Lung fibroblast derived mitogen|_x000D_Scatter factor|_x000D_SF
Species: Human HGF
Assay: 125pg/ml-8000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human HGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA or citrate
Immunogen: sf21, Q32-R494(alpha)&V495-S728(beta)
Gene name: Hepatocyte growth factor / hepatocyte growth factor (hepapoietin A; scatter factor)
Protein name: Hepatocyte growth factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|169|1246|4183|168|1364|4239
Standard deviation|9.8|87.22|267.71|9.91|118.66|313.68
CV(%)|5.8%|7%|6.4%|5.9%|8.7%|7.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 169 | 159 | 165 | 152 | 161 | 6.41 | 3.9% |
| Sample 2 | 1246 | 1103 | 1132 | 1240 | 1180 | 63.61 | 5.3% |
| Sample 3 | 4183 | 3905 | 4308 | 3657 | 4013 | 252.15 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0369-CAP|96-well plate precoated with anti- human HGF antibody|1
EK0369-ST|Lyophilized recombinant human HGF standard|10ng/tube×2
EK0369-DA|Biotinylated anti- human HGF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
