Description
Boster’s human Fibronectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from rabbit specific for Fibronectin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibronectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: CIG|Cold insoluble globulin|Cold-insoluble globulin|Fibronectin 1|FINC|FINC_HUMAN|FN|FN1|FNZ|Migration stimulating factor|MSF|Ugl-Y3
Species: Human Fibronectin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural human Fibronectin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from rabbit|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: from plasma
Gene name: Fibronectin / fibronectin 1
Protein name: Fibronectin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|312|1118|5412|341|1105|4997
Standard deviation|14.35|65.96|308.48|19.43|76.24|349.79
CV(%)|4.6%|5.9%|5.7%|5.7%|6.9%|7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 312 | 333 | 321 | 275 | 310 | 21.67 | 6.9% |
| Sample 2 | 1118 | 1155 | 1256 | 1278 | 1201 | 67 | 5.5% |
| Sample 3 | 5412 | 5418 | 5472 | 5364 | 5416 | 38.27 | 0.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0349-CAP|96-well plate precoated with anti- human Fibronectin antibody|1
EK0349-ST|Lyophilized human Fibronectin standard|10ng/tube×2
EK0349-DA|Biotinylated anti- human Fibronectin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
