Human FGF2 PicoKine ELISA Kit

Brand:
Boster Bio
Storage:

For quantitative detection of human FGF2 in cell culture supernates, serum and plasma (heparin, EDTA).   

 

Available on backorder

SKU: EK0342 Category:

Description

Boster’s human FGF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF2 has been precoated onto 96-well plates. Standards(E.coli,P143-S288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF2 amount of sample captured in plate.


Synonyms: Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB

Species: Human FGF2

Assay: 62.5pg/ml-4000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant human FGF2

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: E.coli,P143-S288

Gene name: Fibroblast growth factor 2 / fibroblast growth factor 2

Protein name: Fibroblast growth factor 2

Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|150|752|1076|135|781|1071
Standard deviation|10.95|54.89|79.62|10.8|62.48|92.1
CV(%)|7.3%|7.3%|7.4%|8%|8%|8.6%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 150 132 141 131 138 7.69 5.5%
Sample 2 752 641 642 667 675 45.37 6.7%
Sample 3 1076 1179 1055 1199 1127 62.59 5.5%

*number of samples for each test n=16.


Catalog number|Description|Quantity
EK0342-CAP|96-well plate precoated with anti- human FGF2 antibody|1
EK0342-ST|Lyophilized recombinant human FGF2 standard|10ng/tubex2
EK0342-DA|Biotinylated anti- human FGF2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4