Description
Boster’s human CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-C119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL24 amount of sample captured in plate.
Synonyms: C C motif chemokine 24|CCL24|Chemokine (C C motif) ligand 24|Chemokine CC Motif Ligand 2|CK beta 6|Ckb 6|Ckb6|Eosinophil chemotactic protein 2|Eotaxin 2 like protein|MPIF 2|MPIF2|Myeloid progenitor inhibitory factor 2|OTTHUMP00000210225|SCYA24|Small inducible cytokine A24|Small inducible cytokine subfamily A (Cys Cys) member 24
Species: Human CCL24
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human CCL24
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, V27-C119
Gene name: C-C motif chemokine 24 / chemokine (C-C motif) ligand 24
Protein name: C-C motif chemokine 24
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|75|209|857|70|228|777
Standard deviation|3.67|13.16|44.56|4.13|16.41|41.18
CV(%)|4.9%|6.3%|5.2%|5.9%|7.2%|5.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 75 | 82 | 86 | 80 | 80 | 3.96 | 4.9% |
| Sample 2 | 209 | 190 | 207 | 207 | 203 | 7.69 | 3.7% |
| Sample 3 | 857 | 1006 | 836 | 933 | 908 | 67.1 | 7.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0331-CAP|96-well plate precoated with anti- human CCL24 antibody|1
EK0331-ST|Lyophilized recombinant human CCL24 standard|10ng/tube×2
EK0331-DA|Biotinylated anti- human CCL24 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
