Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2 • Host: Rabbit • Species Reactivity: (+) Mouse

KCC2 (Phospho-Thr1007) Polyclonal Antibody – 100 µl

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Cayman
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The neuronal-specific potassium chloride cotransporter 2 (KCC2) is a member of the SLC12 family of transporters and is encoded by SLC12A5 in humans.{55121} KCC2 is expressed in mature neurons throughout the CNS and localizes to the soma and dendrite plasma membrane where it mediates chloride ion efflux, maintaining the transmembrane chloride potential.{55122} It consists of 12 transmembrane helices, which contain an extracellular loop subject to N-glycosylation, as well as intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of KCC2 at threonine 1007 (Thr1007) is mediated by lysine-deficient protein kinase 1 (WNK1) and its effectors, STE20/SPS1-related proline-alanine-rich protein kinase (SPAK) and oxidative stress-responsive kinase 1 (OSR1), resulting in reduced KCC2 activity. Phospho-Thr1007 levels are decreased by the KCC2 activator N-ethylmaleimide (NEM) in HEK293 cells.{59146} Point mutation of Thr1007 (T1007E) in KCC2 mimics its phosphorylation and abolishes NEM-induced chloride ion efflux in HEK293 cells. Cayman’s KCC2 (Phospho-Thr1007) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes KCC2 (phospho-Thr1007) at approximately 135 kDa from mouse and rat samples.  

 

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SKU: 29292 - 100 µl Category:

Description

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2 • Host: Rabbit • Species Reactivity: (+) Mouse, Rat • Cross Reactivity: (+) KCC2 (phospho-Thr1007); (-) Unphosphorylated KCC2 • Applications: WB • MW = ~135 kDa

Synonyms: Electroneutral Potassium Chloride Cotransporter 2|Neuronal Potassium Chloride Cotransporter

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2

Formulation: 100 µl of affinity-purified polyclonal antibody from pooled serum

Isotype:

Applications: WB

Origin:

Stability: 365 days

Application|Western Blot||Product Type|Antibodies|Polyclonal Antibodies||Research Area|Neuroscience

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