Antigen: recombinant full-length human caspase-3 • Host: mouse

Caspase-3 Monoclonal Antibody (Clone 31A1067) – 1 ea

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Cayman
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Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis.{18384} The precursor form of all caspases is composed of a prodomain and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation, and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. The gene for caspase-3 also known as Yama, CPP32, and apopain codes for a 32 kDa protein.{8444,18385,18386} Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other caspase-3 substrates include DNA-PK, actin, GAS2, and pro-caspase-6.{6292} Caspase-3 is activated by cleavage events at Asp28/Ser29 (between N-terminal pro-domain) and Asp175/Ser176 (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa.{18385}  

 

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SKU: 13909 - 1 ea Category:

Description

Antigen: recombinant full-length human caspase-3 • Host: mouse, clone 31A1067• Isotype: IgG1κ • Cross Reactivity: (+) human, mouse, and rat caspase-3 • Application(s): IHC (frozen and paraffin-embedded sections) and WB • Caspase-3 cleaves the death substrate PARP to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other caspase-3 substrates include DNA-PK, actin, GAS2, procaspase-6, etc.

Synonyms: Apopain|CPP32|ICE3|Yama

Immunogen: recombinant full-length human caspase-3

Formulation: 100 µg of protein G-purified IgG in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide

Isotype: IgG1κ

Applications: IHC (frozen and paraffin-embedded sections) and WB

Origin:

Stability: 365 days

Application|Immunohistochemistry||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Proteolysis|Cytosolic & Secreted Proteases

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