Peroxiredoxin-6 (Prdx6) is a bifunctional enzyme that has both non-selenium glutathione peroxidase (GPX) and phospholipase A2 (PLA2) activities.{9012} Its PLA2 activity is calcium-independent, functions optimally in acidic (pH = 4) conditions, and facilitates the intracellular processing of surfactant lipids, including dipalmitoylphosphatidylcholine (DPPC).{9012,27803,5680} MJ33 is a selective, reversible inhibitor of the acidic, calcium-independent (ai)PLA2 activity of Prdx6, with optimal inhibition achieved at an MJ33 concentration of 3 mol%.{3982,3927,27802} It blocks the degradation of DPPC in both whole lung and isolated alveolar type II epithelial cells.{27803} MJ33 has been used to examine the role of Prdx6 PLA2 activity in the activation of type 2 NADPH oxidase and in the regulation of Prdx6 PLA2 activity by phosphorylation.{27804,27805}
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A selective, reversible inhibitor of the acidic, calcium-independent (ai)PLA2 activity of Prdx6, with optimal inhibition achieved at an MJ33 concentration of 3 mol%; blocks the degradation of DPPC in both whole lung and isolated alveolar type II epithelial cells
Formal name: phosphoric acid, mono[1-[(hexadecyloxy)methyl]-2-(2,2,2-trifluoroethoxy)ethyl] monomethyl ester, monolithium salt
Synonyms:
Molecular weight: 498.5
CAS: 1007476-63-2
Purity: ≥95%
Formulation: A crystalline solid
The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.{8344,4096,14518} PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.
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The tetracycline repressor (TetR) is a transcriptional regulator which normally binds tightly to its palindromic tetO operator DNA, blocking gene expression.{17508} Tet causes the repressor to dissociate from the DNA, allowing transcription to occur. A novel reverse TetR (revTetR) requires tetracycline as a co-repressor to bind tetO and block transcription.{17509,17510} Anhydrotetracycline (hydrochloride) is a powerful effector in both the tetracycline repressor (TetR) and reverse TetR (revTetR) systems, binding the Tet repressor 35-fold more strongly than Tet.{17508,17511} Moreover, anhydrotetracycline poorly binds the 30S ribosomal subunit, compared to Tet,{17512} so it does not act as a general inhibitor of translation and is a poor antibiotic. Perhaps related to this, the concentration of anhydrotetracycline that inhibits eukaryotic cell growth is more than a 1,000-fold above the dose that alters transcription through TetR.{17508}
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1,2-Dipalmitoyl-sn-glycero-3-PC (DPPC) is a zwitterionic glycerophospholipid commonly used in the formation of lipid monolayers, bilayers, and liposomes for use in a variety of applications.{14480,24261,14478,14479} It has been used in the formation of proteoliposomes for implantation of γ-glutamyl transpeptidase into human erythrocyte membranes.{14478} Incorporation of glycosphingolipid antigens into DPPC-containing liposomes increases the immunogenicity of the antigens in mice.{14479}
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The PtdIn phosphates play an important role in the generation and transduction of intracellular signals.{8344,4096,14518} PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.
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