A steroid-based cationic lipid that can be used to facilitate gene transfection; forms a complex with pDNA and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher; facilitates transfection of the luciferase gene into H1299 cells

2H-Cho-Arg (trifluoroacetate salt) – 5 mg

Brand:
Cayman
CAS:
1609010-59-4
Storage:
-20
UN-No:
Excepted Quantity - 1170 / 3

2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection.{44005} It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin (Item No. 21633) and the caveolae-mediated endocytosis inhibitor genistein (Item No. 10005167), but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml).  

 

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SKU: 25944 - 5 mg Category:

Description

A steroid-based cationic lipid that can be used to facilitate gene transfection; forms a complex with pDNA and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher; facilitates transfection of the luciferase gene into H1299 cells, which can be inhibited by methyl-β-cyclodextrin and genistein; induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml)

Formal name: (3β)-[6-[[(2S)-2-amino-(5α)-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate], cholestan-3-ol, bis(2,2,2-trifluoroacetate)

Synonyms: 

Molecular weight: 886.1

CAS: 1609010-59-4

Purity: ≥95%

Formulation: A solution in ethanol

Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Cell Biology||Research Area|Lipid Biochemistry|Sterol Lipids

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