A bisdeoxy derivative of coelenterazine that produces an emission maximum of 395 nm following conversion by Renilla luciferase; commonly used in BRET2 protocols to evaluate protein-protein interactions

Coelenterazine 400a (hydrochloride) – 500 µg

Brand:
Cayman
CAS:
2320429-05-6
Storage:
-20
UN-No:
Non-Hazardous - /

Coelenterazine 400a is a bisdeoxy derivative of coelenterazine (Item No. 16123) that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h (Item No. 16894), which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.{26296} Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.{26291,26296} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}  

 

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SKU: 25738 - 500 µg Category:

Description

A bisdeoxy derivative of coelenterazine that produces an emission maximum of 395 nm following conversion by Renilla luciferase; commonly used in BRET2 protocols to evaluate protein-protein interactions

Formal name: 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one, monohydrochloride

Synonyms: 

Molecular weight: 427.9

CAS: 2320429-05-6

Purity: ≥95%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area

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