A mitochondrial dye; incorporates into the cardiolipin-rich inner mitochondrial membrane; selectively binds to cardiolipin-containing liposomes over phosphatidylserine- and phosphatidylinositol-containing liposomes in vitro (Kas = 0.002

Acridine Orange 10-nonyl (bromide) – 25 mg

Brand:
Cayman
CAS:
75168-11-5
Storage:
-20
UN-No:
Non-Hazardous - /

Acridine orange 10-nonyl is a mitochondrial dye and derivative of acridine orange (Item No. 14338) that incorporates into the cardiolipin-rich inner mitochondrial membrane.{36790} It selectively binds to cardiolipin-containing liposomes over phosphatidylserine- and phosphatidylinositol-containing liposomes in vitro (Kas = 0.002, 7,000, and 7,000 mM, respectively). Acridine orange 10-nonyl binding to mitochondria is abolished by the mitochondrial uncoupler FCCP (Item No. 15218) in rat cortical astrocytes, neonatal cardiomyocytes, and isolated brain mitochondria, indicating dependence on mitochondrial membrane potential.{36791} Acridine orange 10-nonyl displays excitation/emission maxima of 490/510 nm, respectively, and has been used to quantify cardiolipin content and mitochondrial mass in isolated mitochondria and cellular systems.  

 

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SKU: 25545 - 25 mg Category:

Description

A mitochondrial dye; incorporates into the cardiolipin-rich inner mitochondrial membrane; selectively binds to cardiolipin-containing liposomes over phosphatidylserine- and phosphatidylinositol-containing liposomes in vitro (Kas = 0.002, 7,000, and 7,000 mM, respectively); binding dependent on mitochondrial membrane potential; ex/em = 490/510 nm, respectively; has been used to quantify cardiolipin content and mitochondrial mass in isolated mitochondria and cellular systems,

Formal name: 3,6-bis(dimethylamino)-10-nonyl-acridinium, monobromide

Synonyms:  10-nonyl Acridine Orange

Molecular weight: 472.5

CAS: 75168-11-5

Purity: ≥90%

Formulation: A solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Mitochondrial Biology

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