A high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation; ex/em = 390/523 (free) and 484-496 nm (bound)

bis-ANS (potassium salt) – 5 mg

Brand:
Cayman
CAS:
65664-81-5
Storage:
-20
UN-No:
Non-Hazardous - /

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.{33937} Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.{33952} bis-ANS has an excitation maxima of 390 nm.{14908} It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.{33940} It also potently inhibits microtubule assembly.{33938,33939}  

 

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Description

A high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation; ex/em = 390/523 (free) and 484-496 nm (bound), respectively; labels mechanically damaged neurons; inhibits microtubule assembly

Formal name: 4,4′-bis(phenylamino)-[1,1′-binaphthalene]-5,5′-disulfonic acid, dipotassium salt

Synonyms:  4,4′-Dianilino-1,1′-binaphthyl-5,5′-disulfonic Acid

Molecular weight: 672.9

CAS: 65664-81-5

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Microtubule Dynamics||Research Area|Cell Biology|Cytoskeleton & Motor Proteins||Research Area|Neuroscience

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