A cell-permeant fluorogenic dye used to assess mitochondrial function and cell viability; excitation/emission spectra 515 and 555/575 nm

Tetramethylrhodamine methyl ester (perchlorate) – 5 mg

Brand:
Cayman
CAS:
115532-50-8
Storage:
-20
UN-No:
Non-Hazardous - /

Tetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.{34471,34472,34473} It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization of the mitochondrial membrane, TMRM is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.  

 

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Description

A cell-permeant fluorogenic dye used to assess mitochondrial function and cell viability; excitation/emission spectra 515 and 555/575 nm

Formal name: 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium, perchlorate

Synonyms:  TMR methyl ester|TMRM

Molecular weight: 500.9

CAS: 115532-50-8

Purity: ≥98%

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Application|Immunofluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Mitochondrial Biology

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