A cell-permeant precursor of the ratiometric fluorescent calcium indicator indo-1

Indo-1 AM – 1 mg

Brand:
Cayman
CAS:
112926-02-0
Storage:
-20
UN-No:
Non-Hazardous - /

Indo-1 AM is a cell-permeant acetoxymethyl ester of the ratiometric fluorescent calcium indicator indo-1 (Item Nos. 20418 | 20419). As indo-1 AM enters cells, it is hydrolyzed by intracellular esterases to produce Indo-1. Indo-1 is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium.{30853,23059} Indo-1 is prone to photobleaching, which limits its usefulness in methods involving microscopy.{30853}  

 

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SKU: 20417 - Category:

Description

A cell-permeant precursor of the ratiometric fluorescent calcium indicator indo-1, which is amenable to a single excitation source, typically 349-364 nm light from an argon-ion laser; emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium

Formal name: 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-Indole-6-carboxylic acid, (acetyloxy)methyl ester

Synonyms:  Indo-1 Acetoxymethyl ester

Molecular weight: 1,009.90

CAS: 112926-02-0

Purity: ≥90%

Formulation: A solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Signaling|Calcium Mobilization

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