A cell-permeable version of fura-FF (potassium salt); a low affinity fluorescent calcium indicator; a difluorinated derivative of fura-2; has negligible magnesium sensitivity

Fura-FF AM – 50 µg

Brand:
Cayman
CAS:
348079-12-9
Storage:
-20
UN-No:
Non-Hazardous - /

Fura-FF AM is a cell-permeable acetoxymethyl ester of the fluorescence calcium indicator fura-FF (potassium salt) (Item No. 20415). As fura-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce fura-FF. Fura-FF is a difluorinated derivative of the calcium indicator fura-2 (Item Nos. 19531 | 20414). Unlike, fura-2, fura-FF has negligible magnesium sensitivity, thus reducing interference from this cation.{38261} Fura-FF also has a higher calcium dissociation constant than fura-2 (Kd(calcium) = 6 and 0.14 µM, respectively).{38261,23059} However, the spectral properties of fura-FF and fura-2 are similar with fura-FF displaying excitation/emission spectra of 365/514 nm in the absence of calcium, with a shift to 339/507 nm in the presence of a high calcium concentration.{38262} Low affinity calcium dyes, including fura-FF, are preferred for studying compartments with high concentrations of calcium, such as mitochondria, or in cell systems that have relatively low calcium buffering capacities, such as neuronal dendrites and spines.{31952,31953,31954}  

 

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Description

A cell-permeable version of fura-FF (potassium salt); a low affinity fluorescent calcium indicator; a difluorinated derivative of fura-2; has negligible magnesium sensitivity, unlike fura-2; has a higher calcium dissociation constant than fura-2; ex/em = 365/514, respectively, in the absence of calcium; ex/em = 339/507 in the presence of a high calcium concentration,

Formal name: 2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[6-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-2,3-difluorophenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid(acetyloxy)methyl ester

Synonyms:  Fura-FF Acetoxymethyl ester

Molecular weight: 1,023.80

CAS: 348079-12-9

Purity: ≥90%

Formulation: A solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Signaling|Calcium Mobilization||Research Area|Cell Biology|Mitochondrial Biology

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